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The study used samples that were between two to six years old with insects embedded into it. The researchers were able to use PCR to amplify DNA samples that they retrieved from the resin and then ran the samples on gels to compare with a standard. Another study had provided insight that amplified DNA from these organisms but it did not provide clarity in the methods to make it reproducible.
This past august, Kurt the horse was born. Kurt is a Przewalski’s horse, an endangered species of wild horse that comes from central Asia. There hasn’t been another one seen in the wild since 1969. Kurt was made using a technique known as somatic cell nuclear transfer, a technique where scientists take the nucleus out of a healthy, unfertilized egg and replace it with the nucleus from a somatic cell (any cell other than a reproductive cell) from the animal being cloned.
In a multi-institutional project, scientists have reported the genomes of 363 species of birds. The studied species represents more than 92% of the world’s avian families. The data from the study will advance research on the evolution of birds and aid in the conservation of threatened bird species. Together, the data constitute a rich genomic resource that is now available to the scientific community. The release is a major milestone for the Bird 10,000 Genomes Project (B10K), which aims to sequence the genomes of every avian species on the planet.
Often caused by problems in sperm development, male infertility is solely responsible for 20-70% of all cases of infertility. Scientists from Seoul National University developed a treatment for male infertility through direct intracellular protein delivery using nanotechnology to revive the enzyme activity of PIN1.
The large brains of humans set us apart from other primates, and one specific gene came into play during evolution that made our brains bigger while keeping other primates smaller. Now for the first time, researchers at the Max Planck Institute of Molecular Cell Biology and Genetics have used the same gene, ARHGAP11B, to grow a larger monkey brain. The study may have replicated the moment in evolution where humans became separate from other primates, all due to this one gene.
Researchers from the University of Denmark and Korean Advanced Insitute of Science and Technology have developed a natural transformation based cloning method for DNA fragments. The researchers used a liquid bacterial culture with added DNA substrates that were transformed with robotic automation. The method, coined “ANT cloning” serves as an affordable, high throughput cloning alternative to E.coli.
Harvard Medical School scientists have successfully restored vision in mice by turning back the clock on aged eye cells in the retina and returning youthful gene function. In addition to resetting the cells’ aging clock, the team successfully reversed vision loss in mice with a condition mimicking human glaucoma, a leading cause of blindness worldwide.
Novel T cells genetically engineered by a team at the University of Arizona were able to target and attack pathogenic T cells that cause Type 1 diabetes, which could lead to new immunotherapy treatments.
Cold Spring Harbor Laboratory scientists have developed a new iPhone app capable of connecting with a handheld DNA sequencer, allowing users to create a mobile genetics laboratory. The app, iGenomics, reduces the need for laptops or large field equipment. The iGenomics app was developed by Aspyn Palatnick over a period of eight years, starting when he was only 14-years-old during an internship at the lab.
In a phase 3 clinical trial, researchers from the University of Cambridge, University of Pittsburgh, and other institutions used gene therapy to successfully treat 37 patients suffering from Leber hereditary optic neuropathy (LHON), the most common cause of mitochondrial blindness. In the study, 78% of treated patients treated experienced significant visual improvement in both eyes over 2 years of follow-up. The research suggests that the improvement in vision in untreated eyes could be due to the transfer of viral vector DNA from the injected eye.
By inhibiting certain enzymes involved in abnormal gene transcription, researchers from the University at Buffalo were able to restore memory loss associated with Alzheimer’s disease. They focused on gene changes caused by epigenetic processes such as aging to reverse elevated levels of harmful genes that cause memory deficits in Alzheimer’s disease.
Flying through outer space can take a toll on the body, and during space travel, astronauts experience aging at a faster rate than people on Earth. Recent studies have looked at the health hazards that spending time up in space has had on astronauts, and they revealed that space alters gene function, function of the mitochondria, and the chemical balance in cells.
By growing mouse stem cells in a special gel, researchers from the Max Planck Institute succeeded in producing structure similar to parts of an embryo. The trunk-like structures develop the precursors for neural bone, cartilage, and muscle tissues from cellular clumps within five days. This could allow the scientists to explore the effects of pharmacological agents more effectively—and on a scale that would not be possible in living organisms.
Researchers at Brigham and Women’s Hospital have reported the development of a blood test for a fragment of the protein tau, a hallmark of Alzheimer’s. The test for levels of N-terminal fragment of tau (NT1) has been evaluated in participants of the Harvard Aging Brain Study (HABS), where the authors report that baseline NT1 levels in the blood were highly predictive of the risk of cognitive decline and Alzheimer’s disease dementia in healthy, older adults. The test represents a far less costly and less invasive alternative to image and lumbar punctures.
Researchers from Kyushu University in Japan were able to convert mouse stem cells into oocyte-like cells by activating just eight genes.
We all know those people who can eat what they want without worrying about gaining weight. Thus far, the regulation of fat metabolism has mainly focused on finding genes linked to obesity. However, an international team of researchers have taken a unique approach to discovering genes linked to thinness, or the resistance to weight gain.
Researchers from University of California, Los Angeles developed MitoPunch, a device for transferring the mitochondria and its DNA into mammalian cells. Their approach can help understand genetic components of cells and possibly study the backgrounds of diseases like cancer, diabetes, and metabolic disorders.
Lithium is considered to be the gold standard treatment for bipolar disorder (BD), but nearly 70% of people with BD do not respond to the treatment, leaving them at risk for debilitating, potentially life-threatening mood swings. Researchers at the Salk Institute have found that the culprit may lie in gene activity—or the lack of it. The study could result in a new drug for bipolar disorder as well as a biomarker for lithium non-responsiveness.
Using a genetic screen called transposon-mediated gene activation, researchers from the Benaroya Research Institute searched for natural antiviral mechanisms within cultured human bone cancer cells. Transposons, mobile genetic elements found throughout the genome, can be added to cells to knock out genes they randomly insert into. The team integrated a promoter sequence into the transposons so that they would turn other genes on, while also knocking some genes out. After adding these transposons to human cells, the team introduced a virus engineered to express Ebola glycoprotein, killing most of the cells. Then, they discovered two genes crucial to the cells’ survival: NPC1 and CIITA.
New probes have allowed scientists to see four-stranded DNA interacting with molecules inside living human cells, unraveling its role in cellular processes. DNA typically forms the classic double helix shape we are all familiar with, and while it can also form some more exotic shapes in test tubes, it is very rare to see in real living cells.