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ESET is a protective bundle that includes the solutions for the consumer family windows, mac and Linux operating systems.. Stay protected even in case of data theft or laptop and USB key loss.. Protect your Windows, Mac and Android devices with a single license for the. AV Comparatives Advanced Malware Protection March 2020 Award. United States Court of Appeals
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Sample preparation and extracellular flux assays {#Sec11}
————————————————

*R. rubrum* cells were cultivated in CM medium supplemented with 0.2% glucose as a carbon source, and the assay was performed according to Cheng et al. \[[@CR13]\]. For fermentation monitoring with ionophores, the basal medium with glucose concentration 0.2% (w/v) was also supplemented with appropriate concentration of ionophores (hydroquinidine or tetronasin). The samples were incubated at 30°C in a shaking incubator. Intracellular pH and redox potential were monitored by microelectrodes \[[@CR11], [@CR33]\]. The activity of cytoplasmic lactate dehydrogenase (LDH) was determined by the method of E. F. Merrigan \[[@CR29]\]. Optical density at 600 nm (OD~600~) was measured to determine cell concentrations.

Simultaneous glucose and acetate determination {#Sec12}
———————————————-

The concentration of extracellular glucose and acetate was determined by the automated HPLC analysis system (Shimadzu LC-20 AD) (Shimadzu, Kyoto, Japan). The system consisted of a column (Shim-pack GLC-PHA-H, 4.6 × 250 mm) and a detector (Amine-C18
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